Coding

Part:BBa_K1179051:Design

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)


VC155-Cas9(D10A and H840A mutant)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4137
    Illegal EcoRI site found at 4498
    Illegal PstI site found at 558
    Illegal PstI site found at 2526
    Illegal PstI site found at 2760
    Illegal PstI site found at 3972
    Illegal PstI site found at 4276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4137
    Illegal EcoRI site found at 4498
    Illegal PstI site found at 558
    Illegal PstI site found at 2526
    Illegal PstI site found at 2760
    Illegal PstI site found at 3972
    Illegal PstI site found at 4276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4137
    Illegal EcoRI site found at 4498
    Illegal BglII site found at 565
    Illegal BglII site found at 1502
    Illegal BamHI site found at 1
    Illegal BamHI site found at 2320
    Illegal XhoI site found at 3858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4137
    Illegal EcoRI site found at 4498
    Illegal PstI site found at 558
    Illegal PstI site found at 2526
    Illegal PstI site found at 2760
    Illegal PstI site found at 3972
    Illegal PstI site found at 4276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4137
    Illegal EcoRI site found at 4498
    Illegal PstI site found at 558
    Illegal PstI site found at 2526
    Illegal PstI site found at 2760
    Illegal PstI site found at 3972
    Illegal PstI site found at 4276
    Illegal NgoMIV site found at 3054
    Illegal AgeI site found at 4470
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable

Source

Was constructed by digestion and ligation of BsaI sites on IDT synthesized split venus proteins and the DNA binding mutant Cas9 entry vector with N-terminal BsaI site. The Cas9 comes from the type II prokaryote CRISPR system in Streptococcus pyogenes. The split venus fluorescence protein is a genetic mutant of GFP which comes from the jellyfish Aequorea victoria.

References